Medical-Grade Honey Kills Antibiotic-Resistant Bacteria In Vitro and Eradicates Skin Colonization

Medical-Grade Honey Kills Antibiotic-Resistant Bacteria In Vitro and Eradicates Skin Colonization

عسل پزشکی درجه یک باعث می شود که باکتری های پوستی مقاوم در برابر آنتی بیوتیک را از بین می‌برد.

  • نویسنده: Kwakman PH1, Van den Akker JP, Güçlü A, Aslami H,
  • تعداد صفحات: 6 صفحه
  • سال : 1386 / 2008
  • منتشرکننده: Oxford Academy
  • کلمات کلیدی انگلیسی: antibiotic resistance, bacterial bacteria honey skin microbial colonization
  • کلمات کلیدی فارسی: مقاومت آنتی بیوتیکی ، باکتری های میکروبی پوست، عسل
چکیده

BACKGROUND:

Antibiotic resistance among microbes urgently necessitates the development of novel antimicrobial agents. Since ancient times, honey has been used successfully for treatment of infected wounds, because of its antibacterial activity. However, large variations in the in vitro antibacterial activity of various honeys have been reported and hamper its acceptance in modern medicine.

METHODS:

We assessed the in vitro bactericidal activity of Revamil (Bfactory), a medical-grade honey produced under controlled conditions, and assessed its efficacy for reduction of forearm skin colonization in healthy volunteers in a within-subject-controlled trial.

RESULTS:

With Bacillus subtilis as a test strain, we demonstrated that the variation in bactericidal activity of 11 batches of medical-grade honey was <2-fold. Antibiotic-susceptible and -resistant isolates of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, and Klebsiella oxytoca were killed within 24 h by 10%-40% (vol/vol) honey. After 2 days of application of honey, the extent of forearm skin colonization in healthy volunteers was reduced 100-fold (P < .001), and the numbers of positive skin cultures were reduced by 76% (P < .001).

CONCLUSIONS:

Revamil is a promising topical antimicrobial agent for prevention or treatment of infections, including those caused by multidrug-resistant bacteria.

Honeybee combs: How the circular cells transform into rounded hexagons

Honeybee combs: How the circular cells transform into rounded hexagons

شانه های زنبور عسل: چگونه سلولهای دایره ای به شش ضلعی گرد تبدیل می شوند

  • نویسنده: B.L. Karihaloo , Jian Wang
  • تعداد صفحات: 8 صفحه
  • سال : 1391 / 2013
  • منتشرکننده: Journal of The Royal Society Interface
  • کلمات کلیدی انگلیسی: Honey bee, Honey comb
  • کلمات کلیدی فارسی: شانه عسل، زنبور عسل
چکیده

We report that the cells in a natural honeybee comb have a circular shape at ‘birth’ but quickly transform into the familiar rounded hexagonal shape, while the comb is being built. The mechanism for this transformation is the flow of molten visco-elastic wax near the triple junction between the neighbouring circular cells. The flow may be unconstrained or constrained by the unmolten wax away from the junction. The heat for melting the wax is provided by the ‘hot’ worker bees.

Protective effect of royal jelly on fertility and biochemical parameters in bleomycin-induced male rats

Protective effect of royal jelly on fertility and biochemical parameters in bleomycin-induced male rats

اثر حفاظتی ژل رویال بر باروری و پارامترهای بیوشیمیایی در موشهای صحرایی نر ناشی از بلومایسین

  • نویسنده:Tayebeh Amirshahi Ph.D. Candidate, Gholamreza Najafi Ph.D., Vahid Nejati Ph.D
  • تعداد صفحات: 8 صفحه
  • سال : 1392 / 2014
  • منتشرکننده: Iran J Reprod Med
  • کلمات کلیدی انگلیسی: Bleomycin, Royal jelly, Testis, Testosterone, Rat
  • کلمات کلیدی فارسی: ژل رویال ، بیضه ، تستوسترون ، موش صحرایی
چکیده

Background: Bleomycin (BL) is a glycopeptide antibiotic obtained from the bacterium Streptomyces verticillus which is routinely used for treatment of human cancers. Royal jelly (RJ) is a production from the hypo pharyngeal, mandibular and post cerebral glands of nurse bees. RJ consists of 66% water, 15% sugars, 5% lipids, and 13% proteins, essential amino acids and vitamins.

Objective: The aim of present study was to evaluate protective effect of royal jelly on sperm parameters and malondialdehyde (MDA) production in rat.

Materials and Methods: Forty adult male wistar rats (220±20gr) were randomly divided into 4 groups (n=10). Control group (CG) received normal saline 10 ml/kg twice a week with Intraperitoneal (I.P) for 48 days (0.3 ml/rat(. Royal Jelly group (RJG) received jelly (100 mg/kg daily) for 48 days orally. Bleomycin group (BLG) received BL (10 mg/kg twice a week) with I.P for 48 days. Royal Jelly+ Bleomycin group (RJ+BLG) received royal Jelly (100 mg/kg /day) orally concomitant with BL administration. Sperm count, motility, and viability were investigated and chromatin quality and DNA integrity were also analyzed. Serum testosterone and MDA concentrations were measured as well.

Results: BL caused decline significantly (p<0.05) sperm count, sperm viability, motility as well as testosterone concentration compared to control group while significant (p<0.05) increases in immature sperm, sperm with damaged DNA and MDA concentration were announced in BL in comparison with CG and RJ+BLG. Royal jelly improved Bleomycin-induced toxicity on sperm parameters and testosterone and MDA concentrations.

Conclusion: The present results support the idea that BL adversely affects sperm parameters and MDA and the RJ with antioxidant properties has positive effects on these parameters.

Effect of in ovo Injection of Royal Jelly on Post‐Hatch Growth Performance and Immune Response in Broiler Chickens Challenged with Newcastle Disease Virus

Effect of in ovo Injection of Royal Jelly on Post‐Hatch Growth Performance and Immune Response in Broiler Chickens Challenged with Newcastle Disease Virus

اثر تزریق تزریق ژل رویال بر عملکرد رشد و واکنش ایمنی در جوجه های گوشتی به چالش کشیده شده با ویروس بیماری نیوکاسل

  • نویسنده: Y. Jafari Ahangari , S.R. Hashemi , A. Akhlaghi
  • تعداد صفحات: 6 صفحه
  • سال : 1390 / 2012
  • منتشرکننده: Ijas
  • کلمات کلیدی انگلیسی: broiler, immune system, in ovo injection, performance, royal jelly.
  • کلمات کلیدی فارسی: جوجه گوشتی ، سیستم ایمنی بدن ، تزریق تخم مرغ ، عملکرد ، ژله رویال.
چکیده

The aim of this experiment was to study the effect of in ovo injection of royal jelly (RJ) on growth performance and immune response in broiler chickens challenged with Newcastle disease virus. A total of 200 hatching eggs were divided into four groups of 50 eggs each one using a completely randomized design. The eggs were injected with sterilized sodium bicarbonate buffer (pH=10.4) or RJ (88%) in a 0.3 mL volume into the albumen at d 7 of incubation. A non-injected control and a dry punch control (shell pricked without injecting solution; needle) were included. Chicks hatched from the respective treatment group were reared in floor pens until d 28. The chickens were challenged against Newcastle disease virus on days 7 and 21. On d 21, the chickens emanated from the RJ injected egg had the highest body weight. In ovo injection of RJ had a significant effect on feed intake in broiler chickens during starter period (d 1 to 21) and throughout the trial (d 1 to 28; P<0.05). On d 14, serum antibody titer against newcastle disease virus (NDV) was not significantly different among treatments. On d 14 and 28, heterophil and lymphocyte number and their ratio were affected by in ovo injection. In conclusion, the results of the present study suggested that in ovo injection of RJ on d 7 of incubation exerted a beneficial effect on growth in starter phase and could stimulate feed intake in broiler chickens challenged with Newcastle disease virus, although the antibody titer against NDV was not influenced.

Propolis, royal jelly and pollen from beehive have antibacterial effect on aquatic pathogenic bacterial isolates

Propolis, royal jelly and pollen from beehive have antibacterial effect on aquatic pathogenic bacterial isolates

بره موم ، ژل رویال و گرده حاصل از زنبور عسل دارای خاصیت ضد باکتری بر روی پاتوژن آبزی هستند

  • نویسنده: Shiva Salimi (M.Sc), Nafiseh Sadat Naghavi (Ph.D), Vajiheh Karbasizadeh (Ph.D)​
  • تعداد صفحات: 7 صفحه
  • سال : 1391 / 2013
  • منتشرکننده: IJMCM
  • کلمات کلیدی انگلیسی :Aquatic pathogen, Royal jelly, Propolis, Pollen, Antibacterial effect
  • کلمات کلیدی فارسی: پاتوژن آبزی ، ژل رویال ، بره موم ، گرده ، اثر ضد باکتریایی
چکیده

New approaches for treatment of infectious diseases in aquatic animals have important roles in aquaculture technology progress. In the present study, In vitro effects of different extracts of propolis, royal jelly and pollen obtained from beehives have been investigated on aquatic pathogenic bacterial isolates. The isolated bacteria identified on the basis of their biochemical properties and sequence alignment of the amplified genome fragments. Antimicrobial activities of ethanol extracts of royal jelly, propolis and pollen, and acetone extract of propolis were determined through well diffusion and microdilution methods. The isolated bacteriaidentified as Aeromonas and Vibrio spp., based on biochemical characterization. Alignments of the amplified sequences showed most similarites to Vibrio cholerae and Aeromonas hydrophila. The results obtained from antibacterial effects of extracts showed that the acetone extract of propolis as well as the ethanol extract of royal jelly, had the greatest effect on Aeromonas hydrophila (MIC=25 mg ml-1); and the ethanol extracts of pollen and royal jelly as well as the acetone extract of propolis, showed the greatest effect on Vibrio cholerae (MIC=50 mg ml-1). The results of present in vitro study propose the beehive compounds (royal jelly, propolis and pollen) as powerful natural products to control pathogenic bacteria in aquaculture systems

Study of the distribution and depletion of chloramphenicol residues in bee products extracted from treated honeybee (Apis mellifera L.) colonies

Study of the distribution and depletion of chloramphenicol residues in bee products extracted from treated honeybee (Apis mellifera L.) colonies

بررسی توزیع و کاهش کلرامفنیکلول باقیمانده در محصولات زنبور عسل از زنبور عسل درمان شده

  • نویسنده: Stuart J. Adams, Katharina Heinrich
  • تعداد صفحات: 10 صفحه
  • سال : 1386 / 2008
  • منتشرکننده: Apidologie
  • کلمات کلیدی انگلیسی : chloramphenicol, honey, bees, royal jelly, beeswax
  • کلمات کلیدی فارسی: کلرامفنیکل ، عسل ، زنبورها ، ژل رویال ، موم زنبور عسل
چکیده

Bee colonies were dosed with chloramphenicol (CAP) 1.0 g per hive (single dose in sucrose solution). Samples of honey were then collected at intervals over a 48-week period and samples of royal jelly, beeswax, honeybees and brood collected at intervals over a 12 week period. The mean concentration of CAP in the honey at 7 days after dosing was 26 μg/g, declining to 1.0 μg/g at 332 days. Application of the shook swarm procedure resulted in a mean concentration of CAP in honey of 26 μg/g at 7 days, declining to 0.1 μg/g at 332 days. The mean concentration of CAP in non-honey samples was in the range of 0.5 to 6.8 μg/g, and 0.2 to 3.3 μg/g at 7 days and 56 days, respectively. These results indicate that use of CAP can be detected up to 332 days after dosing even if the shook swarm procedure is used in an attempt to clean the hives. There was no evidence of any significant formation of bound CAP-glucose conjugates in honey.

Potential use of major royal jelly proteins (MRJPs) as molecular markers for royal jelly production in Africanized honeybee colonies

Potential use of major royal jelly proteins (MRJPs) as molecular markers for royal jelly production in Africanized honeybee colonies

مصرف احتمالی پروتئین های ژل رویال اصلی (MRJPs) به عنوان نشانگرهای مولکولی برای تولید ژل رویال در کلونی های زنبور عسل آفریقایی

  • نویسنده: Tatiane Vicente Baitala, Patrícia Faquinello
  • تعداد صفحات: 9 صفحه
  • سال : 1387 / 2009
  • منتشرکننده: Apidologie
  • کلمات کلیدی انگلیسی : Apis mellifera, MRJP-3, genetic variability, polymorphism, EPD
  • کلمات کلیدی فارسی: ژل رویال، زنبور عسل، تنوع ژنتیکی ، پلی مورفیسم
چکیده

The present study determined the genetic variation at mrjps loci (mrjp3, mrjp5 and mrjp8) and evaluated the potential use of MRJPs as molecular markers for higher royal jelly production in Africanized honeybee colonies. The three analyzed loci produced a total of 17 alleles. This high allelic polymorphism indicated these loci could serve as genetic markers. The potential use of MRJPs as molecular markers for royal jelly production was evaluated by analyses of multiple linear regressions with EPD (expected progeny differences) values for royal jelly production. The variance analyses indicated that the mrjp3 repetitive region influenced the genetic value of queen’s offspring for royal jelly production. The determination coefficient (R2) for the significant alleles of the repetitive region of mrjp3 indicated that 36.85% of the EPD variation is explained by the variation of C, D and E alleles. Therefore, these three alleles present a considerable genetic effect on the variation of RJ production.

Changes in composition of royal jelly harvested at different times: consequences for quality standards

Changes in composition of royal jelly harvested at different times: consequences for quality standards

تغییرات در ترکیب ژل رویال در برداشت های مختلف زمانی: عواقب استاندارد های کیفیت

  • نویسنده: H.-Q. Zheng, F.-L. Hu, V. Dietemann
  • تعداد صفحات: 9 صفحه
  • سال : 1389 / 2011
  • منتشرکننده: Apidologie
  • کلمات کلیدی انگلیسی : honeybee, royal jelly, chemical composition, quality standard
  • کلمات کلیدی فارسی: زنبور عسل ، ژل رویال ، ترکیب شیمیایی ، استاندارد کیفیت
چکیده

Most of the studies on royal jelly (RJ) composition or properties as well as quality standards of commercially available royal jelly are based on RJ harvested three days (72 h) after grafting. In China, some beekeepers produce RJ harvested one (24 h) or two (48 h) days after grafting. There is a lack of knowledge about the quality of the royal jelly harvested earlier than 72 h. This study compared 32 colonies for their chemical compositions of RJ harvested at 24, 48 and 72 h after grafting, according to the proportion of moisture, protein, 10-HDA, total sugar and the value of acidity and superoxide dismutase activity. The analysis of RJ samples revealed that the composition varied significantly (for both fresh and dehydrated samples) and on some occasions above and below the range of present Chinese and Swiss standards. The results suggest that harvesting time should be considered when defining new quality standards of RJ. Keywordshoneybee–royal jelly–chemical composition–quality standard

Determination of trans-10-hydroxy-2-decenoic acid content in pure royal jelly and royal jelly products by column liquid chromatography

Determination of trans-10-hydroxy-2-decenoic acid content in pure royal jelly and royal jelly products by column liquid chromatography

تعیین مقدار اسید ترانس 10HDA در ژل رویال خالص و ژل رویال تولید شده با ستون مایع کروماتوگرافی

  • نویسنده: MahmutGenç, AbdurrahmanAslan
  • تعداد صفحات: 4 صفحه
  • سال : 1377 / 1999
  • منتشرکننده: ELSEVIER
  • کلمات کلیدی انگلیسی : Royal jelly, trans-10-Hydroxy-2-decenoic acid
  • کلمات کلیدی فارسی: ژل رویال
چکیده

In this research, several royal jellies and commercial products containing royal jelly were analysed for their trans-10-hydroxy-2-decenoic acid (10-HDA) content by using a column liquid chromatography technique. Ten samples claimed to be pure royal jelly, containing 10-HDA between 0.75 and 2.54%. Seven samples claimed to contain royal jelly as an ingredient which ranged from non-detectable to 0.054%. The technique was found to be rapid with high recovery.

Quality and standardisation of Royal Jelly

Quality and standardisation of Royal Jelly

کیفیت و استاندارد ژل رویال

  • نویسنده: Anna Gloria Sabatini, Gian Luigi Marcazzan
  • تعداد صفحات: 6 صفحه
  • سال : 1387 / 2009
  • منتشرکننده: IBRA
  • کلمات کلیدی انگلیسی : Quality, standardisation, Royal Jelly
  • کلمات کلیدی فارسی: ژل رویال، استاندارد ژل رویال، کیفیت ژل رویال
چکیده

Given the exceptional biological properties attributed to it, royal jelly (RJ) has considerable commercial appeal and is today utilised in many sectors, ranging from the pharmaceutical and food industries to the cosmetic and manufacturing sectors. This has resulted, among other things, in large-scale importation in countries where production is insufficient to meet domestic demand. Research capabilities thus need to be reinforced to permit both a reliable qualitative and quantitative evaluation of the different components and the implementation of analytical tests on commercially available products – RJ on its own or as an additive to new or traditional products – also for the purpose of identifying possible adulteration.