The research was conducted at the apiary of the Faculty of Agriculture, Zanjan University in Zanjan, Iran. Queens were reared in 24 Apis mellifera meda honey bee rearing colonies. The colonies were assigned to 4 grafting larvae age groups; 1 day old larvae, 2 day old larvae, and 3 day old larvae, and the last group reared emergency queen cells.
The groups were divided into the 2 feeding groups: fed additionally and no fed. The effects of the age of the grafted larvae and the effects of supplemental feeding on 9 morphological characteristics of queens were measured. The results showed that the age of the larvae significantly affected the morphological characteristics of reared queens, and thus, their quality. Queens reared from 1 day old larvae were of the highest quality. These queens were significantly heavier (158.83 mg) and had significantly larger spermatheca (0.99 mm3) than queens reared from larvae 2 and 3 days old. Queens from emergency queen cells were of lower quality than queens reared from 1 day old larvae. However, queens from emergency queen cells were of higher quality than queens reared from 3 day old larvae. The supplemental feeding significantly increased most morphological characteristics of the reared queens. The different ages of the larvae did not significantly affect the wing length nor did supplemental feeding affect the wing length.